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What do we do?
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Geographical Areas covered: The laboratory offers comprehensive diagnostic services across the trust which includes Northern General Hospital, Royal Hallamshire Hospital and Western Park Hospital. We also provide complete virology services to the Sheffield Childrens’ Hospital. In addition, the lab acts as a reference centre for the local district general hospitals in Barnsley, Chesterfield, Rotherham and Doncaster. Medical virology cover is provided for Doncaster and Bassettlaw Hospitals. We are the regional stock-holders for VZIG & HBIG.
Platforms: Our automated serology platforms consist of Bayer (Siemens) Centuar (soon to be replaced), Mini-VIDAS and BEP. Molecular platforms include Lightcycler 1 & 2, Roche Amplicor, ABI 7500 and BD SDA / VIPER.
Assays performed In-house:
Serology: HIV 1&2 antibodies (Cenatur, Adaltis, Duo VIDAS Ultra), HAV IgM/Total Ab (Centaur), Complete HBV serology (HBsAg (Centaur & Diasorin), HBcTotal Ab, HBcIgM Ab, HBeAg, HBeAb, HBsAb), HCV Ab (Cenatur, BioRad, RIBA), VZV IgM, IgG (VIDAS & EIA), MMR IgMs & IgGs, CMV Total Ab, IgM, IgG, Avidity, EBV IgM, VCA IgG, EBNA IgG, HSV 1&2 IgG,HTLV 1&2 Ab, CFTs, Parvo IgM, IgG, Legionella Ab, Syphilis (TPPA, VDRL, IgM), Toxoplasma Latex, Toxoplasma IgM, IgG, Mycoplasma GPAT, ASO titres, Anti-DNAse Ab, Helicobacter IgG.
Molecular:
CMV PCR (Roche Lightcycler), HSV PCR (Artus, ABI), VZV PCR (Artus, ABI), EBV PCR (Artus, ABI), Enterovirus PCR (Artus, Lightcycler), HBV PCR (Artus, Lightcycler), HCV PCR (Roche Amplicor), HIV PCR (Roche Amplicor), Respiratory PCRs (Flu A/B, RSV A/B, Rhinovirus, HMPV A/B, PF1-4, Cornavirus, Adenovirus), HCV genotyping, VIPER/SDA /Real-Time PCR for Chlamydia, VIPER/SDA for Gonococcus.
Virus Isolation:
Viral cultures (service to be reviewed, to be wholly or partly withdrawn), Immunoflourescence for respiratory viruses, PCP
Environmental:
Norovirus EIA, Rotavirus EIA (card test), Adenovirus EIA
Main Users:
Infectious Diseases (including HIV, Viral Hepatitis), GUM (including HIV), Haematology (includes Auto/Allo Transplants), Renal Medicine (Dialysis, Transplants), Paediatric Haematology (including Transplants), Cystic Fibrosis Unit, Patients transplanted at the now redundant Lung transplant unit.
Laboratory Sections:
Serology, Automated serology, Molecular, Chlamydias and Virus Isolation
Laboratory location:
We are based at the Northern General Hospital (nearer to Meadowhall, Junction 34 M1). You can find details of hospital location here: (http://www.sth.nhs.uk/patients/2-8.php)
Within the hospital the lab may be difficult to locate. The lab is signposted “PHLS Laboratory” and is found opposite the GT News, on the first floor, above the haematology department. This block is right next to the biochemistry department. All visitors must report to the reception first.
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